activated charcoal agar

Black agar is a nutrient culture dyed with activated charcoal powder. Cultivators use black agar to easily observe mycelial growth.

Black Agar

agar-agar

light malt extract

~1 gram activated charcoal powder

water

Boil

Dry Ingrediants

In an autoclave-safe glass container, add 5g of agar-agar, 7.5 g of light malt extract, and around 1 g of activated charcoal.

Combined

Carefully measure 250ml of the boiling water and pour it into the glass container to combine with the dry ingredients.

Dissolve

Place a filtered lid on the mixture, then shake or swirl the mixture until the dry ingredients begin dissolving.

Protect Lid

Use foil to cover the lid.  This prevents moisture from entering that agar mixture.

Pressure Cooker

Prepare your and securely place the mixture inside a  pressure cooker or autoclave. Seal the lid and cook until the pressure cooker reaches 15-PSI.

Cook at 15-PSI

Cook the agar for 45-minutes after the pressure cooker reaches 15-PSI.

Depressurize

Once the time elapses, turn off the heat and allow the pressure cooker to return to zero PSI before cracking the lid open. When ready, remove the agar and place it in your work area.

Monitor Temperature

The agar is ready to pour once it reaches a temp between 116-122°F (46-50°C).

Ready to Pour

Now, you are ready to pour your mixture into petri dishes. You must follow the sterilization protocol for the next part.  It is a good idea to prep while the agar mixture is cooking.

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Top 1 activated charcoal agar recipes from across the web

The most viewed activated charcoal agar recipes by Recipe Cart users, saved from over 6,000 sites. Or, go to our Recipe Search Engineic_arrow_forward_48px

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Black Agar

The most viewed activated charcoal agar recipes by Recipe Cart users, saved from over 6,000 sites. Or, go to our Recipe Search Engine